Proteome analysis of peroxisomes from dark-treated senescent Arabidopsis leaves
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چکیده
منابع مشابه
Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms.
We have established a protocol for the isolation of highly purified peroxisomes from mature Arabidopsis thaliana leaves and analyzed the proteome by complementary gel-based and gel-free approaches. Seventy-eight nonredundant proteins were identified, of which 42 novel proteins had previously not been associated with plant peroxisomes. Seventeen novel proteins carried predicted peroxisomal targe...
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Organelles in spinach leaves were isolated in sucrose gradients by isopycnic centrifugation. The peroxisomal fraction was the only cellular site for two different and irreversible aminotransferases which utilized glyoxylate as the amino acceptor in the formation of glycine. These were a serine:glyoxylate aminotransferase with a specific activity of 1.54 pmoles X min-1 X mg-1 peroxisomal protein...
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The biosynthetic pathway of Chl, probably the most important pigment in the biosphere, has been studied in considerable detail and is mentioned in plant physiology textbooks. Oddly enough, however, the breakdown of Chl is barely mentioned. Yet, as pointed out by Hendry et al. (1987), an estimated one billion tons of Chl are broken down every year, seemingly without leaving a trace. The only enz...
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ژورنال
عنوان ژورنال: Journal of Integrative Plant Biology
سال: 2018
ISSN: 1672-9072
DOI: 10.1111/jipb.12670